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The divergent restoration effects of Lactobacillus strains in antibiotic-induced dysbiosis

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  • Accepted manuscript

    Accepted author manuscript, 933 KB, PDF-document

    Embargo ends: 29/10/19

DOI

Authors

Abstract

To evaluate functions of Lactobacillus strains, isolated from fermented food, in restoration of ampicillin-induced disruption based on mucosal barrier, gut microbial community and metabolome analyses, three Lactobacillus strains, L. plantarum CGMCC12436 (LacP), L. casei CGMCC 12,435 (LacC) and L. rhamnosus strain GG (LacG) were individually administered to ampicillin-pretreated mice. All three strains significantly restored concentrations of endotoxin and diamine oxidase to control levels. Linear discriminate analysis based on 16S rRNA sequencing of faecal bacteria revealed that the restoration of microbial communities by Lactobacillus strains was more effective than natural restoration. Correlation analysis between microbiota and metabolites indicated that, the higher level of acetate in LacC group was positively correlated with increased relative abundance of Citrobacter, Bifidobacterium and S24-7. Furthermore, LacC down-regulated the expression of NF-κB p65 and modulated the ampicillin-induced inflammatory responses. The LacC strain could particularly attenuate ampicillin-induced disruption by optimisation of microbial taxa and enhancement of acetate and butyrate production.

Details

Original languageEnglish
Pages (from-to)142-152
Number of pages11
JournalJournal of Functional Foods
Volume51
Early online date29 Oct 2018
DOIs
Publication statusPublished - Dec 2018
Peer-reviewedYes
Externally publishedYes

Keywords

    Research areas

  • Functional food, Gut dysbiosis, Immunity, Metabolites, Microbial ecology

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