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The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA

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@article{d57a5c5202b240ae874891c7f1253506,
title = "The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA",
abstract = "NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 {\AA} (1 {\AA}=0.1 nm) resolution. The architecture of the protein is that of a 40 {\AA} {\textquoteleft}nanowire{\textquoteright} in which the five haems are positioned within 6 {\AA} of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein–protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2–NrfB2 heterotetrameric complex.",
author = "TA Clarke and JA Cole and DJ Richardson and AM Hemmings",
year = "2007",
month = may,
day = "23",
doi = "10.1042/BJ20070321",
language = "English",
volume = "406",
pages = "19--30",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "1",

}

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TY - JOUR

T1 - The crystal structure of the pentahaem c-type cytochrome NrfB and characterization of its solution-state interaction with the pentahaem nitrite reductase NrfA

AU - Clarke, TA

AU - Cole, JA

AU - Richardson, DJ

AU - Hemmings, AM

PY - 2007/5/23

Y1 - 2007/5/23

N2 - NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 Å (1 Å=0.1 nm) resolution. The architecture of the protein is that of a 40 Å ‘nanowire’ in which the five haems are positioned within 6 Å of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein–protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2–NrfB2 heterotetrameric complex.

AB - NrfB is a small pentahaem electron-transfer protein widely involved in the respiratory reduction of nitrite or nitric oxide to ammonia, processes that provide energy for anaerobic metabolism in many enteric bacteria and also serve to detoxify these reactive nitrogen species. The X-ray crystal structure of Escherichia coli NrfB is presented at 1.74 Å (1 Å=0.1 nm) resolution. The architecture of the protein is that of a 40 Å ‘nanowire’ in which the five haems are positioned within 6 Å of each other along a polypeptide scaffold. During nitrite reduction, the physiological role of NrfB is to mediate electron transfer to another pentahaem protein, NrfA, the enzyme that catalyses periplasmic nitrite or nitric oxide reduction. Protein–protein interaction studies suggest NrfA and NrfB can form a 20-haem NrfA2–NrfB2 heterotetrameric complex.

U2 - 10.1042/BJ20070321

DO - 10.1042/BJ20070321

M3 - Article

VL - 406

SP - 19

EP - 30

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 1

ER -

ID: 856299