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Novel real-time PCR assays for the specific detection of human infective Cryptosporidium species

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Abstract

Cryptosporidium is a protozoan parasite causing gastrointestinal illness. Drinking waterborne outbreaks have been caused by C. hominis, C. parvum and C. cuniculus. Molecular detection techniques already exist for Cryptosporidium and usually target housekeeping genes. We set ourselves the task to identify species-specific genes. These genes are likely to be involved in host parasite interaction and virulence. Three subtelomeric species-specific putative virulence factor genes (Cops-2, Chos-1 and Chos-2) were identified in silico and used to develop novel real-time PCR assays. Our results show that Chos-2 is a suitable target for probe-based assays for the specific detection of C. hominis and C. cuniculus (two very closely related species) and that Cops-2 is a suitable target for specific detection of C. parvum.

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Original languageEnglish
Pages (from-to)395-399
Number of pages5
JournalVirulence
Volume7
Issue number4
Early online date18 Feb 2016
DOIs
StatePublished - 2016
Peer-reviewedYes

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© 2016 The Author(s). Published with license by Taylor & Francis© Maha Bouzid, Kristin Elwin, Johanna L. Nader, Rachel M. Chalmers, Paul R. Hunter, and Kevin M. Tyler This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. Permission is granted subject to the terms of the License under which the work was published. Please check the License conditions for the work which you wish to reuse. Full and appropriate attribution must be given. This permission does not cover any third party copyrighted material which may appear in the work requested.

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