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MinION Analysis and Reference Consortium: Phase 1 data release and analysis

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  • Camilla L.C. Ip
  • Matthew Loose
  • John R. Tyson
  • Mariateresa de Cesare
  • Bonnie L. Brown
  • Miten Jain
  • Richard M. Leggett
  • David A. Eccles
  • Vadim Zalunin
  • John M. Urban
  • Paolo Piazza
  • Rory J. Bowden
  • Benedict Paten
  • Solomon Mwaigwisya
  • Elizabeth M. Batty
  • Jared T. Simpson
  • Terrance P. Snutch
  • Ewan Birney
  • David Buck
  • Sara Goodwin
  • Hans J. Jansen
  • Hugh E. Olsen

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Abstract

The advent of a miniaturized DNA sequencing device with a high-throughput contextual sequencing capability embodies the next generation of large scale sequencing tools. The MinION™ Access Programme (MAP) was initiated by Oxford Nanopore Technologies™ in April 2014, giving public access to their USB-attached miniature sequencing device. The MinION Analysis and Reference Consortium (MARC) was formed by a subset of MAP participants, with the aim of evaluating and providing standard protocols and reference data to the community. Envisaged as a multi-phased project, this study provides the global community with the Phase 1 data from MARC, where the reproducibility of the performance of the MinION was evaluated at multiple sites. Five laboratories on two continents generated data using a control strain of Escherichia coli K-12, preparing and sequencing samples according to a revised ONT protocol. Here, we provide the details of the protocol used, along with a preliminary analysis of the characteristics of typical runs including the consistency, rate, volume and quality of data produced. Further analysis of the Phase 1 data presented here, and additional experiments in Phase 2 of E. coli from MARC are already underway to identify ways to improve and enhance MinION performance.

Details

Original languageEnglish
Article number1075
JournalF1000Research
Volume4
Issue number1075
DOIs
Publication statusPublished - 15 Oct 2015
Peer-reviewedYes

Bibliographic note

Date of Acceptance: 23/11/2015 © 2015 Ip CLC et al. This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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