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Mimicking Angiogenesis in vitro: Three-dimensional Co-culture of Vascular Endothelial Cells and Perivascular Cells in Collagen Type I Gels

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Authors

  • Markus Auler
  • Lena Pitzler
  • Ernst Poschl
  • Zhigang Zhou
  • Bent Brachvogel

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Abstract

Angiogenesis defines the process of formation of new vascular structures form existing blood vessels, involved during development, repair processes like wound healing but also linked to pathological changes. During angiogenic processes, endothelial cells build a vascular network and recruit perivascular cells to form mature, stable vessels. Endothelial cells and perivascular cells secrete and assemble a vascular basement membrane and interact via close cell-cell contacts. To mimic these processes in vitro we have developed a versatile three-dimensional culture system where perivascular cells (PVC) are co-cultured with human umbilical cord vascular endothelial cells (HUVEC) in a collagen type I gel. This co-culture system can be used to determine biochemical and cellular processes during neoangiogenic events with a wide range of analyses options.

Details

Original languageEnglish
Article numbere2247
JournalBIO-Protocol
Volume7
Issue number8
DOIs
Publication statusPublished - 20 Apr 2017
Peer-reviewedYes

Keywords

    Research areas

  • Endothelial cells, Perivascular cells, Pericytes, Angiogenesis, Collagen gel

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