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Mendelian Inheritance Pattern and High Mutation Rates of Microsatellite Alleles in the Diatom Pseudo-nitzchia multistriata

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Authors

  • Sylvie V.M Tesson
  • Christophe Legrand
  • Cock van Oosterhout
  • Marina Montresor
  • Wiebe H.C.F Kooistra
  • Gabriele Procaccini

Organisational units

Abstract

The diatom Pseudo-nitzschia multistriata exhibits a diplontic life cycle composed of an extensive phase of vegetative cell division and a brief phase of sexual reproduction. To explore genotypic stability, we genotyped seven polymorphic microsatellite loci in 26 monoclonal strains over 3–16 months in a culture maintenance regime. Moreover, to assess inheritance patterns of the microsatellite alleles, we genotyped 246 F1 strains resulting from four mating experiments between parental strains of know genotype. Results generally conformed expectations according to Mendelian inheritance patterns, but deviations were detected indicating mutations during sexual reproduction. A total of forty-two mutations were detected in the clonal cultures over time. Microsatellites with more core-repeats accumulated mutations faster. The mutation rate varied significantly across loci and strains. A binomial mass function and a computer simulation showed that the mutation rate was significantly higher during the first months of culture (μ≈3×10-3 per locus per cell division) and decreased to μ≈1×10-3 in the strains kept for 16 months. Our results suggest that genetic mutations acquired in both the vegetative phase and sexual reproduction add to the allelic diversity of microsatellites, and hence to the genotypic variation present in a natural population.

Details

Original languageEnglish
Pages (from-to)89-100
Number of pages12
JournalProtist
Volume164
Issue number1
Early online date28 Aug 2012
DOIs
Publication statusPublished - Jan 2013
Peer-reviewedYes

Keywords

    Research areas

  • Asexual and sexual reproduction, diatom, Mendelian inheritance, microsatellites, mutation accumulation lines, mutation rate

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