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Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics.

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Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics. / Dimov, Ivan K; Garcia-Cordero, Jose L; O'Grady, Justin; Poulsen, Claus R; Viguier, Caroline; Kent, Lorcan; Daly, Paul; Lincoln, Bryan; Maher, Majella; O'Kennedy, Richard; Smith, Terry J; Ricco, Antonio J; Lee, Luke P.

In: Lab on a Chip, Vol. 8, No. 12, 2008, p. 2071-2078.

Research output: Contribution to journalArticle

Harvard

Dimov, IK, Garcia-Cordero, JL, O'Grady, J, Poulsen, CR, Viguier, C, Kent, L, Daly, P, Lincoln, B, Maher, M, O'Kennedy, R, Smith, TJ, Ricco, AJ & Lee, LP 2008, 'Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics.', Lab on a Chip, vol. 8, no. 12, pp. 2071-2078. https://doi.org/10.1039/b812515e

APA

Dimov, I. K., Garcia-Cordero, J. L., O'Grady, J., Poulsen, C. R., Viguier, C., Kent, L., Daly, P., Lincoln, B., Maher, M., O'Kennedy, R., Smith, T. J., Ricco, A. J., & Lee, L. P. (2008). Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics. Lab on a Chip, 8(12), 2071-2078. https://doi.org/10.1039/b812515e

Vancouver

Author

Dimov, Ivan K ; Garcia-Cordero, Jose L ; O'Grady, Justin ; Poulsen, Claus R ; Viguier, Caroline ; Kent, Lorcan ; Daly, Paul ; Lincoln, Bryan ; Maher, Majella ; O'Kennedy, Richard ; Smith, Terry J ; Ricco, Antonio J ; Lee, Luke P. / Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics. In: Lab on a Chip. 2008 ; Vol. 8, No. 12. pp. 2071-2078.

Bibtex- Download

@article{59bb4217ac8344429f3b4f90127558c8,
title = "Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics.",
abstract = "We demonstrate the first integrated microfluidic tmRNA purification and nucleic acid sequence-based amplification (NASBA) device incorporating real-time detection. The real-time amplification and detection step produces pathogen-specific response in < 3 min from the chip-purified RNA from 100 lysed bacteria. On-chip RNA purification uses a new silica bead immobilization method. On-chip amplification uses custom-designed high-selectivity primers and real-time detection uses molecular beacon fluorescent probe technology; both are integrated on-chip with NASBA. Present in all bacteria, tmRNA (10Sa RNA) includes organism-specific identification sequences, exhibits unusually high stability relative to mRNA, and has high copy number per organism; the latter two factors improve the limit of detection, accelerate time-to-positive response, and suit this approach ideally to the detection of small numbers of bacteria. Device efficacy was demonstrated by integrated on-chip purification, amplification, and real-time detection of 100 E. coli bacteria in 100 microL of crude lysate in under 30 min for the entire process.",
author = "Dimov, {Ivan K} and Garcia-Cordero, {Jose L} and Justin O'Grady and Poulsen, {Claus R} and Caroline Viguier and Lorcan Kent and Paul Daly and Bryan Lincoln and Majella Maher and Richard O'Kennedy and Smith, {Terry J} and Ricco, {Antonio J} and Lee, {Luke P}",
year = "2008",
doi = "10.1039/b812515e",
language = "English",
volume = "8",
pages = "2071--2078",
journal = "Lab on a Chip",
issn = "1473-0197",
publisher = "Royal Society of Chemistry",
number = "12",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - Integrated microfluidic tmRNA purification and real-time NASBA device for molecular diagnostics.

AU - Dimov, Ivan K

AU - Garcia-Cordero, Jose L

AU - O'Grady, Justin

AU - Poulsen, Claus R

AU - Viguier, Caroline

AU - Kent, Lorcan

AU - Daly, Paul

AU - Lincoln, Bryan

AU - Maher, Majella

AU - O'Kennedy, Richard

AU - Smith, Terry J

AU - Ricco, Antonio J

AU - Lee, Luke P

PY - 2008

Y1 - 2008

N2 - We demonstrate the first integrated microfluidic tmRNA purification and nucleic acid sequence-based amplification (NASBA) device incorporating real-time detection. The real-time amplification and detection step produces pathogen-specific response in < 3 min from the chip-purified RNA from 100 lysed bacteria. On-chip RNA purification uses a new silica bead immobilization method. On-chip amplification uses custom-designed high-selectivity primers and real-time detection uses molecular beacon fluorescent probe technology; both are integrated on-chip with NASBA. Present in all bacteria, tmRNA (10Sa RNA) includes organism-specific identification sequences, exhibits unusually high stability relative to mRNA, and has high copy number per organism; the latter two factors improve the limit of detection, accelerate time-to-positive response, and suit this approach ideally to the detection of small numbers of bacteria. Device efficacy was demonstrated by integrated on-chip purification, amplification, and real-time detection of 100 E. coli bacteria in 100 microL of crude lysate in under 30 min for the entire process.

AB - We demonstrate the first integrated microfluidic tmRNA purification and nucleic acid sequence-based amplification (NASBA) device incorporating real-time detection. The real-time amplification and detection step produces pathogen-specific response in < 3 min from the chip-purified RNA from 100 lysed bacteria. On-chip RNA purification uses a new silica bead immobilization method. On-chip amplification uses custom-designed high-selectivity primers and real-time detection uses molecular beacon fluorescent probe technology; both are integrated on-chip with NASBA. Present in all bacteria, tmRNA (10Sa RNA) includes organism-specific identification sequences, exhibits unusually high stability relative to mRNA, and has high copy number per organism; the latter two factors improve the limit of detection, accelerate time-to-positive response, and suit this approach ideally to the detection of small numbers of bacteria. Device efficacy was demonstrated by integrated on-chip purification, amplification, and real-time detection of 100 E. coli bacteria in 100 microL of crude lysate in under 30 min for the entire process.

U2 - 10.1039/b812515e

DO - 10.1039/b812515e

M3 - Article

VL - 8

SP - 2071

EP - 2078

JO - Lab on a Chip

JF - Lab on a Chip

SN - 1473-0197

IS - 12

ER -

ID: 825126