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A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana

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A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana. / Kirkham, Amy; Richthammer, Patrick; Schmidt, Katrin; Wustman, Martin; Maeda, Yoshiaki; Hedrich, Rene; Brunner, Eike; Tanaka, Tsuyoshi; van Pee, Karl-Heinz; Falciatore, Angela; Mock, Thomas.

In: The ISME Journal, Vol. 11, No. 11, 11.2017, p. 2452-2464.

Research output: Contribution to journalArticle

Harvard

Kirkham, A, Richthammer, P, Schmidt, K, Wustman, M, Maeda, Y, Hedrich, R, Brunner, E, Tanaka, T, van Pee, K-H, Falciatore, A & Mock, T 2017, 'A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana', The ISME Journal, vol. 11, no. 11, pp. 2452-2464. https://doi.org/10.1038/ismej.2017.100

APA

Kirkham, A., Richthammer, P., Schmidt, K., Wustman, M., Maeda, Y., Hedrich, R., Brunner, E., Tanaka, T., van Pee, K-H., Falciatore, A., & Mock, T. (2017). A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana. The ISME Journal, 11(11), 2452-2464. https://doi.org/10.1038/ismej.2017.100

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Author

Kirkham, Amy ; Richthammer, Patrick ; Schmidt, Katrin ; Wustman, Martin ; Maeda, Yoshiaki ; Hedrich, Rene ; Brunner, Eike ; Tanaka, Tsuyoshi ; van Pee, Karl-Heinz ; Falciatore, Angela ; Mock, Thomas. / A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana. In: The ISME Journal. 2017 ; Vol. 11, No. 11. pp. 2452-2464.

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@article{2299be635134458aaf9712b982bddc82,
title = "A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana",
abstract = "Diatoms contribute 20% of global primary production and form the basis of many marine food webs. Although their species diversity correlates with broad diversity in cell size, there is also an intraspecific cell-size plasticity due to sexual reproduction and varying environmental conditions. However, despite the ecological significance of the diatom cell size for food-web structure and global biogeochemical cycles, our knowledge about genes underpinning the size of diatom cells remains elusive. Here, a combination of reverse genetics, experimental evolution and comparative RNA8 sequencing analyses enabled us to identify a previously unknown genetic control of cell size in the diatom Thalassiosira pseudonana. In particular, the targeted deregulation of the expression of the cell-wall protein silacidin caused a significant increase in valve diameter. Remarkably, the natural downregulation of the silacidin gene transcript due to experimental evolution under low temperature also correlated with cell-size increase. Our data give first evidence for a genetically controlled regulation of cell size in Thalassiosira pseudonana and possibly other centric diatoms as they also encode the silacidin gene in their genomes.",
author = "Amy Kirkham and Patrick Richthammer and Katrin Schmidt and Martin Wustman and Yoshiaki Maeda and Rene Hedrich and Eike Brunner and Tsuyoshi Tanaka and {van Pee}, Karl-Heinz and Angela Falciatore and Thomas Mock",
year = "2017",
month = nov,
doi = "10.1038/ismej.2017.100",
language = "English",
volume = "11",
pages = "2452--2464",
journal = "The ISME Journal",
issn = "1751-7362",
publisher = "Nature Publishing Group",
number = "11",

}

RIS (suitable for import to EndNote) - Download

TY - JOUR

T1 - A role for the cell-wall protein silacidin in cell size of the diatom Thalassiosira pseudonana

AU - Kirkham, Amy

AU - Richthammer, Patrick

AU - Schmidt, Katrin

AU - Wustman, Martin

AU - Maeda, Yoshiaki

AU - Hedrich, Rene

AU - Brunner, Eike

AU - Tanaka, Tsuyoshi

AU - van Pee, Karl-Heinz

AU - Falciatore, Angela

AU - Mock, Thomas

PY - 2017/11

Y1 - 2017/11

N2 - Diatoms contribute 20% of global primary production and form the basis of many marine food webs. Although their species diversity correlates with broad diversity in cell size, there is also an intraspecific cell-size plasticity due to sexual reproduction and varying environmental conditions. However, despite the ecological significance of the diatom cell size for food-web structure and global biogeochemical cycles, our knowledge about genes underpinning the size of diatom cells remains elusive. Here, a combination of reverse genetics, experimental evolution and comparative RNA8 sequencing analyses enabled us to identify a previously unknown genetic control of cell size in the diatom Thalassiosira pseudonana. In particular, the targeted deregulation of the expression of the cell-wall protein silacidin caused a significant increase in valve diameter. Remarkably, the natural downregulation of the silacidin gene transcript due to experimental evolution under low temperature also correlated with cell-size increase. Our data give first evidence for a genetically controlled regulation of cell size in Thalassiosira pseudonana and possibly other centric diatoms as they also encode the silacidin gene in their genomes.

AB - Diatoms contribute 20% of global primary production and form the basis of many marine food webs. Although their species diversity correlates with broad diversity in cell size, there is also an intraspecific cell-size plasticity due to sexual reproduction and varying environmental conditions. However, despite the ecological significance of the diatom cell size for food-web structure and global biogeochemical cycles, our knowledge about genes underpinning the size of diatom cells remains elusive. Here, a combination of reverse genetics, experimental evolution and comparative RNA8 sequencing analyses enabled us to identify a previously unknown genetic control of cell size in the diatom Thalassiosira pseudonana. In particular, the targeted deregulation of the expression of the cell-wall protein silacidin caused a significant increase in valve diameter. Remarkably, the natural downregulation of the silacidin gene transcript due to experimental evolution under low temperature also correlated with cell-size increase. Our data give first evidence for a genetically controlled regulation of cell size in Thalassiosira pseudonana and possibly other centric diatoms as they also encode the silacidin gene in their genomes.

U2 - 10.1038/ismej.2017.100

DO - 10.1038/ismej.2017.100

M3 - Article

VL - 11

SP - 2452

EP - 2464

JO - The ISME Journal

JF - The ISME Journal

SN - 1751-7362

IS - 11

ER -

ID: 112962955