γδ T Cells Shape Preimmune Peripheral B Cell Populations

Research output: Contribution to journalArticle



  • Yafei Huang
  • Andrew Getahun
  • Ryan A. Heiser
  • Thiago O. Detanico
  • Katja Aviszus
  • Greg A. Kirchenbaum
  • Tamara L. Casper
  • Chunjian Huang
  • M. Kemal Aydintug
  • Simon R. Carding
  • Koichi Ikuta
  • Hua Huang
  • Lawrence J. Wysocki
  • John C. Cambier
  • Rebecca L. O’Brien
  • Willi K. Born

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We previously reported that selective ablation of certain γδ T cell subsets, rather than removal of all γδ T cells, strongly affects serum Ab levels in nonimmunized mice. This type of manipulation also changed T cells, including residual γδ T cells, revealing some interdependence of γδ T cell populations. For example, in mice lacking Vγ4(+) and Vγ6(+) γδ T cells (B6.TCR-Vγ4(-/-)/6(-/-)), we observed expanded Vγ1(+) cells, which changed in composition and activation and produced more IL-4 upon stimulation in vitro, increased IL-4 production by αβ T cells as well as spontaneous germinal center formation in the spleen, and elevated serum Ig and autoantibodies. We therefore examined B cell populations in this and other γδ-deficient mouse strains. Whereas immature bone marrow B cells remained largely unchanged, peripheral B cells underwent several changes. Specifically, transitional and mature B cells in the spleen of B6.TCR-Vγ4(-/-)/6(-/-) mice and other peripheral B cell populations were diminished, most of all splenic marginal zone (MZ) B cells. However, relative frequencies and absolute numbers of Ab-producing cells, as well as serum levels of Abs, IL-4, and BAFF, were increased. Cell transfers confirmed that these changes are directly dependent on the altered γδ T cells in this strain and on their enhanced potential of producing IL-4. Further evidence suggests the possibility of direct interactions between γδ T cells and B cells in the splenic MZ. Taken together, these data demonstrate the capability of γδ T cells of modulating size and productivity of preimmune peripheral B cell populations.


Original languageEnglish
Pages (from-to)217-31
Number of pages15
JournalJournal of Immunology
Issue number1
Early online date18 Nov 2015
Publication statusPublished - 1 Jan 2016

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